This abstract is related to a seminar delivered by Dr. Platt sing Isolation of Mesenchymal root cells from kids ‘s dentitions and geographic expedition of their function in tissue fix…..
Induced Pluripotent Stem Cells
Induced Plenteous Stem Cells Applied to the Held of Regenerative Medicine Imagine having the opportunity to travel back in time with the power to alter the outcome of the future. As pleasing as this may sound, such occurrences just do not seem realistic or even possible in today’s world. Although, if we take a step back and look into time travel on a smaller scale, at the cellular level, it is indeed possible to revert to an earlier stage of life.
Specifically focusing on terminally differentiated anatomic cells, scientists are now able to induce plenipotentiary thanks to the findings from Sir John B. Gordon, and Shinny Yamaha. Findings from Cordon’s paper inform us that all cells in an organism contain the same genetic Information. The difference in gene expression leads to one cell type over another. This Implies that somatic cells should have the ability to give rise to a variety of cell types under the appropriate conditions because all of the cells have the same DNA content.
Findings from Handyman’s paper tell us that the factors responsible from the maintenance of allurements in early embryos and embryonic stem cells also are responsible for inducing plenipotentiary in somatic cells. The factors from Handyman’s discovery are COT-3/4, KILL, SOX, and c-NYC; these four transcription factors work together to effectively induce plenipotentiary and have greatly advanced the technological applications of genetic reprogramming. The field of regenerative medicine has especially benefited from the genetic reprogramming advances.
One of the main goals of regenerative medicine is to restore structures of damaged tissues as well as to restore functions of damaged organs . A major application for regenerative medicine Is In the field of cardiovascular medicine. The use of regenerative medicine for cardiovascular disease treatment Is appealing because It Is much less Invasive that transplantation and open-heart surgery. In order to determine which combination transcription factors are able to create normal cell fates from the damaged cardiac tissue, induced plenteous stem cells are an intricate part of the drug screening technique often used.
The drugs capable of inducing plenipotentiary and repairing the damaged tissue have the potential to treat cardiovascular disease n human patients. Alternative options for repairing damaged tissue include transplantation of new organs from donors. Cardiovascular diseases continue to account for the leading cause of death in American. Due to the high frequency of deaths caused by heart malfunctions in society, researchers are constantly trying to discover new ways for treatment and prevention.
Despite the alternative methods used to treat cardiovascular diseases, the use of Induced plenteous stem cells for regenerative medicine Is overall beneficial to the field of cardiovascular medicine cause It Is a less Invasive option that can effectively restore wounded cardiac tissue by replacing cardiologists and reducing occurrences of fibrosis (Upon, 2011). Cardiovascular repair than other options, such as transplanting an entire heart. Before regenerative medicine had the clinical potential it currently possesses, scientists first needed to have a comprehensive understanding of the heart and its development processes.
Muscle tissue in the heart is referred to as cardiac muscle. One cell type that encompasses cardiac muscle is the cardiology. Each mature dull cardiology only contains a single, unique nucleus and expresses cardiac transcriptions factors, which allows for their calcium ion handling and contractile properties (Upon, 2011). Normally, these adult cardiologists cannot regenerate once damaged (Upon). This leads to the malfunction or loss of function within the heart, causing many of the heart conditions prevalent in society.
Studies show that induced plenteous stem cells can differentiate into the three electroencephalographic phenotypes of cardiologists: nodal, trial, and ventricular (Upon, 2011). In a injunction human heart, nodal cardiologists are found at the bottom, trial cardiologists pump blood in, and ventricular cardiologists pump blood out to the lungs and the rest of the body. Each phenotype shows that suppositories can successfully increase the rate of contraction while ceremonially can decrease the rate of contraction (Upon).
Ellen Peon’s review paper explains that the function of cardiologists in the heart is to perform the contraction for blood flow. More importantly, it explains that the presence of specific chemicals, suppositories and ceremonially, effects the speed of contraction. Peon’s findings about the specifics of heart contractions are extremely beneficial information in terms of regenerative medicine applied to cardiovascular diseases because a heart that is uncontrollably pumping too fast or too slow has the potential to be regulated simply by adding either suppositories or ceremonially concentrations to the environment.
Both chemicals could be studied further to determine if a drug design involving the two is reasonable for treatment. Even though using induced plenteous stem cells to generate heart tissue is less invasive, there are drawbacks to be looked onto further by researchers. In the review paper, Upon notes that cardiology’s derived from induced plenteous cells are immature functionally and structurally. This is problematic because at immature stages, the electrical properties of the cardiologists are similar to that of those of heart failure.
It was also observed the the derived cardiologists were about ten times smaller than the normal adult cardiologists. Most importantly, the safety and practical worth of the induced cardiology’s is unknown because there is not a lot of information about the amphibology of these cells. Downsides noted in Samurai’s paper include the large-scale preparation that goes into creating these induced cardiologists and the elimination of undifferentiated induced plenteous stem cells from generating the cardiology’s.
With all that being said, there is promising potential for using regenerative medicine as a less invasive means to treat cardiovascular diseases. An alternate method used to bypass these downfalls is the cell-sheet technique. This technique involves harvesting a sheet of undamaged cells and transplanting them directly on top of the injured organ. The idea is that the undamaged cells will essentially promote recovery of the organ’s wounded cells. Kumara and others illustrate the efficacy of induced plenteous stem cell derived cardiology sheets. Improve cardiac function (Kumara, 2011).
Chemic cardiopulmonary is when there is a lack of oxygen supply to the heart muscle tissue, causing measurable deterioration of it function. Sheets of cardiologists capable of restoring tissue after being deteriorated is a feasible option for treating chemic cardiopulmonary. However, this is still invasive for the patient and tissue is still be removed from one action and transplanted to another. Inducing regenerative cardiologists is still a much less invasive option to restore cardiac tissue. As previously mentioned, there is not a lot of regenerative potential for heart tissue once it has been wounded.
One of the reasons for this is due to the activation of cardiac fibroblasts (Song 2012). A cardiac fibroblast is a particular heart cell type that is responsible for maintaining the structural integrity of connective tissues. Activation of these fibroblasts leads to cardiac fibrosis (Song, 2012), which is simply the formation of excess connective issue in the hearth. It is the fibrosis that interferes with regeneration of cardiac cells causing a number of problems like the loss of contractile function and the increased susceptibility to arrhythmias (Song, 2012).
But because most of the cells of the heart are in fact cardiac fibroblasts, they are a potential regenerative medicinal source of cardiac function restoration. One major success of regenerative medicine as it relates to cardiovascular medicine is the repair of heart tissue by reprogramming non- mystery with cardiac transcription factors performed by Kuhn Song along with there researchers. Song’s paper shows that GATE, Hand, MFC, and TPTB are able to reprogram mouse cardiac fibroblasts into contracting cardiac-like mystery in vitro and in vivo.
The discovery of these essential cardiac inducing factors can act as the platform for answering more fundamental research questions in the future. Data from Songs research illustrate that the expression of these four transcription factors enhances cardiac function while decreeing adverse ventricular remodeling following infarction. Findings from this study shows that the efficiency of reprogramming to induced cardiac-like mystery using GATE, Hand, MFC, and TPTB is comparable to the reprogramming of induced plenteous stem cells by the Yamaha factors (Song).
The method of retrovirus transduction was used to incorporate the transcription factors into the genome of the non-moseyed in order to bring the cells back to a plenteous state. Although viral and retrovirus transduction is a convenient method to incorporate new DNA sequences, there are drawbacks that may be adversely effecting outcomes of the induced plenteous cells. There are a couple of key disadvantages using viruses and vectors to incorporate DNA into a host’s genome. One disadvantage is the genomic integration that occurs with retrovirus vectors.
Genomic integration involves inserting DNA sequences directly into the host’s cells DNA. The problem is that vectors integrate near the starting point of transcription causing either enhance transcription more than normal or this can disrupt transcriptions. The results from both outcomes are altered expression due to the technique used rather than the genetic information present. The likelihood of residual transgender expression is another disadvantage to using vectors as a means of incorporating new DNA sequences.
Residual transgender expression is when genetic material from one organism remains after it is transferred leading to the protein synthesis with the contents of the lingering DNA. Residual transgender expressions, research has been done to find alternative methods that are as practical in a clinical setting. A successful alternative to retrovirus transduction involves virus-free integration methods. In relation to cardiovascular medicinal applications, cardiologists have been successfully derived from virus- free induced plenteous cells.
Shish Meta and others demonstrate that virus-free induced plenteous stem cells are able to differentiate into cardiologists with the characteristic cardiac-specific properties. The induced cardiologists showed think and thin filaments of muscle proteins, as expected to be seen normal adult cardiologists (Meta, 2011). Additionally, the induced cardiologists expressed calcium ion handling and ion channel proteins, which further confirms the heart tissue development. Overall, the virus-free methods are preferred over retrovirus vector methods in clinical settings because the outcomes have less harmful potential in vivo.
Despite some of the critical downfalls discussed, it is clear that the use of induced plenteous stem cells for regenerative medicine is ultimately useful to the field of cardiovascular medicine because it is a less invasive option that effectively restores wounded cardiac tissue by inducing cardiologists and reducing occurrences of fibrosis (Upon, 2012). Peon’s paper shows that in the presence of suppositories and ceremonially, cardiologists contraction rates are affected. Drug designers working to create heart-regulatory drugs to control abnormalities in retreats now have more information to work with.
Additionally, researchers are able to use this information as a platform for additional research to understand how to control irregularities in cardiology contractions. The use of regenerative medicine for cardiovascular disease treatments is an emerging technological strategy that holds great potential for the future of human health.